Dynamic metabolomics is a vital tool for industrial biotechnology. Sample preparation approaches dramatically influence data quality and, ultimately, interpretation and conclusions from metabolome experiments. However, standardized protocols for highly reproducible metabolic datasets are limited.
Recently, the research group led by Prof. SUN Jibin and Prof. ZHENG Ping presented an optimized liquid chromatography-tandem mass spectrometry-based metabolomics methodology for Aspergillus niger, an important industrial workhorse. It was found that fast filtration with liquid nitrogen was more suitable for cell quenching, due to its minimal disruption to cell integrity, and improved intracellular metabolite recovery. For intracellular metabolite extraction, acetonitrile/water (1:1, v/v) at -20°C, combined with boiling ethanol extraction protocols, achieved unbiased metabolite profiling. This improved methodology has been applied to unveil the dynamic metabolite profile of one citrate over-producing A. niger isolate under citrate fermentation. This study delivers a highly standardized and improved metabolomics methodology and paves the way for systems metabolic engineering of the biotechnologically important fungi.
Intracellular metabolite profiling of intermediates involved in central metabolic pathway in A. niger during citrate fermentation (Image by TIB)
Contact：Prof. SUN Jibin and Prof. ZHENG Ping
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
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