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XUE Chaoyou

XUE Chaoyou, Ph.D. 

Investigator, TIB, Tianjin, China 







2013.08-2017.12         Ph.D. Iowa State University, USA

2010.09-2013.07         M.S. Tianjin University China

2006.09-2010.07         B.S. Northwest A&F University, China

Professional Experience

2021.01-current       Investigator, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China           

2017.12-2020.12     Postdoctoral Researcher, Columbia University, USA

Research Interests

We mainly use Total Internal Reflection Fluorescence Microscopy (TIRFM) for single molecule fluorescence imaging, such as smFRET and DNA curtains. We aim to use single molecule fluorescence imaging techniques to understand the molecular mechanisms of CRISPR-Cas system and improve CRISPR enzymes’ activities and specifies, to decipher how double-strand breaks (DSBs) are repaired through homologous recombination, to elucidate the defense mechanisms against foreign DNA and DSBs repair mechanisms in non-model organisms and establish gene manipulation systems in non-model organisms, to develop novel tools for whole-genome synthesis.

Academic Awards

2015     Outstanding Thesis Award (M.S.) of Tianjin

2016     The Best Graduate Student Poster Award of the 11th Annual Stupka Symposium

2017     Iowa State University Research Excellent Award

2020     Journal of Biological Chemistry Early Career Reviewer

Publication Records

  1. Xue C, Greene E C. Factors favoring HDR Choice in Response to CRISPR/Cas9 induced-DSB. Trends in Genetics. (invited).
  2. Xue C*, Molnarvova L*, Steinfeld J, Zhao W, Ma C, Spirek M, Kaniecki K, Kwon Y, Belá? O, Boulton S, Sung P, Greene E C, Krejci L. Single-Molecule visualization of human RECQ5 interactions with single-stranded DNA recombination intermediates. Nucleic Acids Res. 2021. 1(49):285-305.
  3. Meir A*, Kong M*, Xue C, Greene E C. DNA curtains shed Light on Complex Molecular Systems During Homologous Recombination. J. Vis. Exp, e61320, 2020. (in press).
  4. Kong M*, Cutts E*, Pan D, Beuron F, Kaliyappan T, Xue C, Morries E, Musacchio A, Vannini A, Greene E C. Human condensing I and II drive extensive ATP-dependent compaction of nucleosome-bound DNA. Mol Cell. 2020, 79:1-16.
  5. Jia N*, Unciuleac M*, Xue C, Greene E C, Patel D, Shuman S. Structures and single-molecule kinetics analysis of the motor-nuclease AdnAB illuminate the mechanism of DNA double-strand break resection. PNAS. 2019. 116 (49): 24507-24516.
  6. Xue C, Daley J, Xue X, Steinfeld J, Kwon Y, Sung P, Greene E C. Single-Molecule visualization of human BLM helicase as it acts upon double- and single-stranded DNA substrates. Nucleic Acids Res. 2019, 1.1035.
  7. Yan Z*, Xue C*, Kumar S*, Crickard J B, Yu Yang, Wang W, Pham N, Sung P, Greene E C, Ira G. Rad52 regulates resection at DNA double strand break ends. Mol Cell. 2019, 1:1-13.
  8. Crickard J B, Xue C, Wang W, Kwon Y, Sung P, Greene E C. The RecQ helicase Sgs1 drives ATP-dependent disruption of Rad51 filaments. Nucleic Acids Res, 2019, 47(9): 4694-4706.
  9. Xue C, Wang W, Crickard J B, Moevus C J, Kwon Y, Sung P, Greene E C. Regulatory control of Sgs1 and Dna2 during eukaryotic DNA end resection. PNAS, 2019, 116 (23), 6091-6100.
  10. Xue C, Greene E C. New roles for RAD52 in DNA repair. Cell Res, 2018, 28:1127-1128.
  11. Phan PT, Schelling M, Xue C, Sashital D G. Fluorescence-based methods for measuring target interference by CRISPR-Cas systems. Methods Enzymol, 2019, 616, 61-85.
  12. Xue C, Sashital D G. Mechanisms of Type IE and IF CRISPR-Cas Systems in Enterobacteriaceae, EcoSal Plus, 2019, 8(2).
  13. Xue C, Zhu Y, Hawk B, Yin L, Shin Y K, Sashital D G. Real-time observation of target search by the CRISPR surveillance complex Cascade. Cell reports, 2017, 21(13), 3717-3727.
  14. Xue C, Whitis N, Sashital D G. Conformational control of Cascade interference and priming activities in CRISPR immunity. Mol Cell, 2016, 64(4), 826-834.
  15. Xue C, Seetharam A S, Musharova O, Severinov K, Brouns S J, Severin A J, & Sashital D G. CRISPR interference and priming varies with individual spacer sequences. Nucleic Acids Res, 2015, 43(22): 10831-10847.
  16. Xue C, Duan Y, Zhao F, & Lu W. Stepwise increase of spinosad production in Saccharopolyspora spinosa by metabolic engineering. Biochem Eng J, 2013, 72: 90-95.
  17. Xue C*, Zhang X*, Yu Z, Zhao F, Wang M, & Lu W. Up-regulated spinosad pathway coupling with the increased concentration of acetyl-CoA and malonyl-CoA contributed to the increase of spinosad in the presence of exogenous fatty acid. Biochem Eng J, 2013, 81: 47-53.
  18. Zhang X, Xue C, Zhao F, Li D, Yin J, Zhang C, Caiyin Q, Lu W. Suitable extracellular oxidoreduction potential inhibit rex regulation and effect central carbon and energy metabolism in S. spinosa. Microb Cell Fact, 2014,13:98.
  19. Zhao F, Xue C, Wang M, Wang X, Lu W. A comparative metabolomics analysis of S. spinosa WT, WH124, and LU104 revealed metabolic mechanisms correlated with increases in spinosad yield. Biosci Biotech Biochem, 2013, 77(8): 1661-1668.
  20. Zhu L, Yang X, Xue C, Chen Y, Qu L, & Lu W. Enhanced rhamnolipids production by Pseudomonas aeruginosa based on a pH stage-controlled fed-batch fermentation process. Bioresour Technol, 2012, 117: 208-213.
  21. Yang X, Zhu L, Xue C, Chen Y, Qu L, & Lu W. Recovery of purified lactonic sophorolipids by spontaneous crystallization during the fermentation of sugarcane molasses with Candida albicans O-13-1. Enzyme Microb Technol, 2012, 51(6): 348-353. 


  1. Wenyu Lu, Xiaoyang Wang, Chuanbo Zhang, Chaoyou Xue. Reconstruction and validation of genome-scale metabolic network of Saccharopolyspora spinosa. CN. 201310756418.0
  2. Wenyu Lu, Chaoyou Xue, Xiangmei Zhang. The construction and application of genetically engineered Saccharopolyspora spinose to the production of spinosad. CN. 201310756049.5
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