2022
58. Ming H, Yuan B, Qu G*, Sun Z*. Engineering the activity of Amine Dehydrogenase in the Asymmetric Reductive Amination of Hydroxyl Ketones. Catal. Sci. Technol., 2022, DOI: 10.1039/D2CY00391K.
57. Jiang Y#, Li X#, Liu B, Tong F, Qu G*, Sun Z*. Engineering the hydrogen transfer pathway of an alcohol dehydrogenase to increase activity by rational enzyme design. Mol. Catal., 2022, 530, 112603.
56. Wang P, Han X, Liu X*, Lin R, Chen Y, Sun Z*, Zhang W*. Synthesis of Enantioenriched Sulfoxides by an Oxidation-Reduction Enzymatic Cascade. Chem. Eur. J.. 2022, e202201997.
55. Lu J, Wang Z, Jiang Y, Sun Z*, Luo W*. Modification of the substrate specificity of leucine dehydrogenase by site-directed mutagenesis based on biocomputing strategies. Syst. Microbiol. and Biomanuf., 2022. https://doi.org/10.1007/s43393-022-00116-5.
54. Sang X, Tong F, Zeng Z*, Wu M, Yuan B, Sun Z, Sheng X, Qu G, Alcalde M, Hollmann F, Zhang W*. A Biocatalytic Platform for the Synthesis of Enantiopure Propargylic Alcohols and Amines. Org. Lett., 2022, 24(23), 4252-4257.
53. Qin Z, Zhang X, Sang X, Zhang W, Qu G*, Sun Z*. Carboxylic acid reductases enable intramolecular lactamization reactions. Green Synth. Catal., 2022. https://doi.org/10.1016/j.gresc.2022.05.009.
52. Wang J#, Qu G#, Xie L, Gao C, Jiang Y, Zhang YPJ, Sun Z*, You C*. Engineering of a thermophilic dihydroxy-acid dehydratase toward glycerate dehydration for in vitro biosystems. Appl. Microbiol. Biotechnol., 2022, 106, 3625-3637.
51. Li Y, Zhang P, Sun Z, Li H, Ge R, Sheng X, Zhang W*. Peroxygenase-Catalyzed Selective Synthesis of Calcitriol Starting from Alfacalcidol. Antioxidants, 2022, 11, 1044.
50. Zhang J#, Liao D#, Chen R#, Zhu F, Ma Y, Gao L, Qu G, Cui C, Sun Z*, Lei X*, Gao S*. Tuning an Imine Reductase for the Asymmetric Synthesis of Azacycloalkylamines by Concise Structure-Guided Engineering. Angew. Chem. Int. Ed., 2022. e202201908.
49. Song S#, Jiang Y#, Chen R, Su W, Liang W, Yang D, Li J, Zhang W, Gao S, Yuan B*, Qu G*, Sun Z*. Whole-cell Biotransformation of Penicillin G by a Three-enzyme Co-expression System with Engineered Deacetoxycephalosporin C Synthase. ChemBioChem, 2022, e202200179.
48. Yang D, Su W, Jiang Y, Gao S, Li X, Qu G*, Sun Z*. Biosynthesis of β-lactam nuclei in yeast. Metab. Eng., 2022, 72, 56-65.
47. 毕悦欣,蒋迎迎,覃宗敏,曲戈*,孙周通*.醇脱氢酶金属离子绑定位点的可替换性. 生物工程学报, 2022, 38(04),1518-1526.
46. Jiang Y#, Qu G#, Sheng X, Tong F, Sun Z*. Unraveling the mechanism of enantio-controlling switches of an alcohol dehydrogenase toward sterically small ketone. Catal. Sci. Technol., 2022, 12, 1777-1787.
45. Qu G, Bi Y, Liu B, Li JK, Han X, Liu W, Jiang Y, Qin Z, Sun Z*. Unlocking the Stereoselectivity and Substrate Acceptance of Enzymes: Proline Induced Loop Engineering Test. Angew. Chem. Int. Ed., 2022, 61 (1), e202110793.
44. Bi H#, Qu G#, Wang S, Zhuang Y, Sun Z*, Liu T*, Ma Y. Biosynthesis of a Rosavin Natural Product in Escherichia coli by Glycosyltransferase Rational Design and Artificial Pathway Construction. Metab. Eng., 2022, 69, 15-25.
43. Dong C#, Qu G#, Guo J#, Wei F, Gao S, Sun Z, Jin L, Sun X, Rochaix JD, Miao Y*, Wang R*. Rational design of geranylgeranyl diphosphate synthase enhances carotenoid production and improves photosynthetic efficiency in Nicotiana tabacum. Science Bulletin, 2022, 67(3), 315-327.
2021
42. Tong F#, Qin Z#, Wang H#, Jiang Y, Li J, Ming H, Qu G, Xiao Y*, Sun Z*. Biosynthesis of Chiral Amino Alcohols via an Engineered Amine Dehydrogenase in E. coli. Front. Bioeng. Biotechnol., 2021, 9: 778584.
41. Wu R, Yu Y, Wang Y, Wang YZ, Song H, Ma C, Qu G, You C, Sun Z, Zhang W, Li A, Li CM*, Yong YC*, Zhu Z*. Wastewater-powered high-value chemical synthesis in a hybrid bioelectrochemical system. iScience, 2021, 103401.
40. 张发光, 曲戈, 孙周通*, 马军安*. 从化学合成到生物合成—天然产物全合成新趋势. 合成生物学, 2021, 2(5), 674-696.
39. 张武元*, 袁波, 曲戈, 孙周通. 光促酶催化反应设计及生物合成应用. 生物学杂志 (J. Biol.), 2021, 38(5), 1-11. 特约综述
38. Li Y, Yuan B, Sun Z, Zhang W*. C–H bond functionalization reactions enabled by photobiocatalytic cascades. Green Synth. Catal., 2021, 2 (3), 267-274.
37. Chen Q, Guo M, Bi Y, Qu G, Sun Z, Wang Y*, Luo G. Whole-cell biocatalytic synthesis of S-(4-chlorophenyl)-(pyridin-2-yl) methanol in a liquid–liquid biphasic microreaction system. Bioresource Technol., 2021, 330: 125022.
36. Li J#, Qu G#, Shang N, Chen P, Men Y, Liu W, Mei Z, Sun Y*, Sun Z*. Near-perfect control of the regioselective glucosylation enabled by rational design of glycosyltransferases. Green Synth. Catal., 2021, 2, 45-53.
2020
35. Acevedo-Rocha, CG*, Hollmann F*, Sanchis J*, Sun Z*. A Pioneering Career in Catalysis: Manfred T. Reetz. ACS Catal., 2020, 10, 15123-15139. (invited)
34. Wang H#, Qu G#, Li JK#, Ma JA, Guo J, Miao Y, Sun Z*. Data mining of amine dehydrogenases for the synthesis of enantiopure amino alcohols. Catal. Sci. Technol., 2020. 10, 5945-5952.
33. 蒋迎迎,曲戈,孙周通*. 机器学习助力酶定向进化. 生物学杂志 (J. Biol.), 2020, 37(4), 1-11. 特约综述
32. Mei Z#, Zhang K#, Qu G, Li JK, Liu B, Ma JA, Tu R*, Sun Z*. A High-Throughput Fluorescence Assay for Ketone Detection and its Applications in Enzyme Mining and Protein Engineering. ACS Omega, 2020, 5, 13588-13594.
31. Qu G#, Li A#, Acevedo-Rocha CG#, Sun Z*, Reetz MT*. The Crucial Role of Methodology Development in Directed Evolution of Selective Enzymes. Angew. Chem. Int. Ed., 2020, 59, 13204-13231. (invited)
30. Chen J#, Fan F#, Qu G#, Tang J, Xi Y, Bi C, Sun Z*, Zhang X*. Identification of Absidia orchidis steroid 11β-hydroxylation system and its application in engineering Saccharomyces cerevisiae for one-step biotransformation to produce hydrocortisone. Metab. Eng., 2020, 57, 31-42.
2019
29. 张锟#,曲戈#,刘卫东,孙周通. 工业酶结构与功能的构效关系. 生物工程学报 (Chin. J. Biotech.), 2019, 35(10): 1806-1818. (邀请综述)
28. Qu G#, Liu B#, Zhang K, Jiang Y, Zhai C, Guo J, Wang R, Miao Y, Sun Z*. Computer-assisted engineering of the catalytic activity of a carboxylic acid reductase. J. Biotechnol., 2019, 306, 97-104. (invited)
27. Qu G#, Liu B#, Zhang K, Jiang Y, Zhai C, Guo J, Wang R, Miao Y, Sun Z*. Computer-assisted engineering of the catalytic activity of a carboxylic acid reductase. J. Biotechnol., 2019, doi.org/10.1016/j.jbiotec.2019.09.006.
26. Qu G#, Li A#, Acevedo-Rocha CG#, Sun Z*, and Reetz MT*. The Crucial Role of Methodology Development in Directed Evolution of Selective Enzymes. Angew. Chem. Int. Ed., 2019, DOI: 10.1002/anie.201901491.
25. Li A#, Qu G#, Sun Z*, and Reetz MT*. Statistical Analysis of the Benefits of Focused Saturation Mutagenesis in Directed Evolution Based on Reduced Amino Acid Alphabets. ACS Catal., 2019, 9, 7769-7778.
24. Liu B#, Qu G#, Li J, Fan W, Ma JA, Xu Y, Nie Y*, and Sun Z*. Conformational Dynamics-Guided Loop Engineering of an Alcohol Dehydrogenase: Capture, Turnover and Enantioselective Transformation of Difficult-to-Reduce Ketones. Adv. Synth. Catal., 2019, 361, 3182-3190.
23. Qu G#, Liu B#, Jiang Y, Nie Y, Yu H, and Sun Z*. Laboratory evolution of an alcohol dehydrogenase towards enantioselective reduction of difficult-to-reduce ketones. Bioresour. Bioprocess. 2019, 6(1):18.
22. Sun Z*, Liu Q, Qu G, Feng Y*, Reetz MT*. The Utility of B-Factors in Protein Science: Interpreting Rigidity, Flexibility and Internal Motion and Engineering Thermostability. Chem. Rev., 2019, 119, 1626-1665.
21. Qu G#, Fu M#, Zhao L, Liu B, Liu P, Fan W, Ma JA, Sun Z*. Computational Insights into the Catalytic Mechanism of Bacterial Carboxylic Acid Reductase. J. Chem. Inf. Model., 2019, 59,832-841.
20. Dai Z#, Liu Y#, Sun Z#, Wang D, Qu G, Ma X, Fan F, Zhang L, Li S, Zhang X*. Identification of a novel cytochrome P450 enzyme that catalyzes the C-2α hydroxylation of pentacyclic triterpenoids and its application in yeast cell factories. Metab. Eng., 2019, 51, 70-78.
19. Sun Z, Reetz MT*. CHAPTER 12 Controlling the Regio- and Stereoselectivity of Cytochrome P450 Monooxygenases by Protein Engineering. In Dioxygen-dependent Heme Enzymes, The Royal Society of Chemistry: 2019; pp 274-291.
2018
18. Li A, Sun Z, Reetz MT*. Solid-Phase Gene Synthesis for Mutant Library Construction: The Future of Directed Evolution? ChemBioChem, 2018, 19 (19), 2023-2032.
17. Qu G, Guo J, Yang D, Sun Z*. Biocatalysis of carboxylic acid reductases: Phylogenesis, Catalytic Mechanism and Potential Applications. Green Chem., 2018, 20(4), 777-792.
16. Qu G, Lonsdale R, Yao P, Li G, Liu B, Reetz MT*, Sun Z*. Methodology Development in Directed Evolution: Exploring Options When Applying Triple Code Saturation Mutagenesis. ChemBioChem, 2018, 19, 239-246.
15. Sun Z#, Wu L#, Bocola M, Chan H.C. S, Lonsdale R, Kong X.-D, Yuan S*, Zhou J*, Reetz MT*. Structural and Computational Insight into the Catalytic Mechanism of Limonene Epoxide Hydrolase Mutants in Stereoselective Transformations. J. Am. Chem. Soc., 2018, 140 (1), 310-318.
14. Li A, Acevedo-Rocha CG, Sun Z, Cox T, Xu J, Reetz MT*. Beating Bias in Directed Evolution of Proteins: Combining High-Fidelity On-Chip Solid-Phase Gene Synthesis with Efficient Gene Assembly for Combinatorial Library Construction. ChemBioChem, 2018, 19(3), 221-228.
13. Acevedo-Rocha CG*, Sun Z*, Reetz MT*. Clarifying the Difference between Iterative Saturation Mutagenesis as a Rational Guide in Directed Evolution and OmniChange as a Gene Mutagenesis Technique. ChemBioChem, 2018, 19 (24), 2542-2544.
12. Yang J#, Zhu Y#, Qu G, Zeng Y, Tian C, Dong C, Men Y, Dai L, Sun Z*, Sun Y*, Ma Y. Biosynthesis of dendroketose from different carbon sources using in vitro and in vivo metabolic engineering strategies. Biotechnol. Biofuels, 2018, 11, 290.
Before 2016
11. Sun Z#, Salas PT#, Siirola E#, Lonsdale R#, Reetz MT*. Exploring productive sequence space in directed evolution using binary patterning versus conventional mutagenesis strategies. Bioresour. Bioprocess, 2016, 3:44, 1-8.
10.Li A, Ilie A, Sun Z, Lonsdale R, Xu JH,Reetz MT*. Whole-Cell-Catalyzed Multiple Regio- and Stereoselective Functionalizations in Cascade Reactions Enabled by Directed Evolution. Angew. Chem. Int. Ed., 2016, 55, 12026 -12029.
9. Sun Z#, Li G#, Ilie A#, Reetz MT*. Exploring the substrate scope of mutants derived from the robust alcohol dehydrogenase TbSADH. Tetrahedron Letters, 2016, 57, 3648-3651.
8. Sun Z, Lonsdale R, Li G, Reetz MT*. Comparing Different Strategies in Directed Evolution of Enzyme Stereoselectivity: Single- versus Double-Code Saturation Mutagenesis. ChemBioChem, 2016, 17, 1865-1872.
7. Li G#, Zhang H#, Sun Z, Liu X*, Reetz MT*. Multiparameter Optimization in Directed Evolution: Engineering Thermostability, Enantioselectivity and Activity of an Epoxide Hydrolase. ACS. Catal., 2016, 6, 3679–3687.
6. Sun Z, Wikmark Y, B?ckvall J-E*, Reetz MT*. New Concepts for Increasing the Efficiency in Directed Evolution of Stereoselective Enzymes. Chem. Eur. J., 2016, 22, 5046-5054.
5. Sun Z, Lonsdale R, Ilie A, Li G, Zhou J, Reetz MT*. Catalytic Asymmetric Reduction of Difficult-to-Reduce Ketones: Triple Code Saturation Mutagenesis of an Alcohol Dehydrogenase. ACS. Catal., 2016, 6, 1598-1605.
4. Sun Z, Lonsdale R, Wu L, Li G, Li A, Wang J, Zhou J*, Reetz MT*. Structure-Guided Triple-Code Saturation Mutagenesis: Efficient Tuning of the Stereoselectivity of an Epoxide Hydrolase. ACS. Catal., 2016, 6, 1590-1597.
3. Sun Z, Ilie A, Reetz MT*. Towards the Production of Universal Blood by Structure-guided Directed Evolution of Glycoside Hydrolases. Angew. Chem. Int. Ed., 2015, 54, 9158-9160.
2. Sun Z, Lonsdale R, Kong XD, Xu JH, Zhou J*, Reetz MT*. Reshaping an Enzyme Binding Pocket for Enhanced and Inverted Stereoselectivity: Use of Smallest Amino Acid Alphabets in Directed Evolution. Angew. Chem. Int. Ed., 2015, 54, 12410-12415.
1. Sun Z, Ning Y, Liu L, Liu Y, Sun B, Jiang W, Yang C, Yang S*. Metabolic engineering of the L-phenylalanine pathway in Escherichia coli for the production of S- or R-mandelic acid. Microb. Cell Fact., 2011, 10:71. “highly accessed”
Invited Book chapters:
3. Qu G, Sun Z*. In silico prediction methods for site-saturation mutagenesis In “Enzyme Engineering: Methods and Protocols” of Methods in Molecular Biology, Humana Press 2022, 2397, pp49-69.
2. Qu G, Sun Z*, Reetz MT*. Iterative Saturation Mutagenesis for Semi-rational Enzyme Design, Protein Engineering: Tools and Applications, Wiley 2021, pp105-132.
1. Sun Z, Reetz MT*. CHAPTER 12 Controlling the Regio- and Stereoselectivity of Cytochrome P450 Monooxygenases by Protein Engineering. In Dioxygen-dependent Heme Enzymes, The Royal Society of Chemistry: 2019; pp 274-291.