Microbial metabolic engineering assisted with systems biology toolboxes. Highlyinterestto establish key technologies for construction ofefficient microbial cell factories with focus onproduction of bulk chemicals and high value-added fine chemicals.

1.Wang Y*, Cheng H, Liu Y, Liu Y, Wen Y, Zhang K, Ni X, Gao N, Fan L, Zhang Z, Liu J, Chen J, Wang L, Guo Y, Zheng P*, Wang M*, Sun J and Ma Y. In-situ generation of large numbers of genetic combinations for metabolic reprogramming via CRISPR-guided base editing. Nat Commun. 2021, 12:678. https://doi.org/10.1038/s41467-021-21003-y

2.Sun X#, Li Q#, Wang Y#, Zhou W, Guo Y, Chen J, Zheng P*, Sun J*, Ma Y. Isoleucyl-tRNA synthetase mutant based whole-cell biosensor for high-throughput selection of isoleucine overproducers.Biosens Bioelectron. 2021,172.

3.Fan L, Wang Y*, Qian J, Gao N, Zhang Z, Ni X, Sun L, Yuan Q, Zheng P* and Sun J.Transcriptome analysis reveals the roles of nitrogen metabolism and sedoheptulose bisphosphatase pathway in methanol-dependent growth of Corynebacterium glutamicum.Microb Biotechnol. 2021.

4.Wang Y*, Liu Y, Zheng P, Sun J and Wang M*. Microbial Base Editing: A Powerful Emerging Technology for Microbial Genome Engineering. Trends Biotechnol. 2020.

5.Wang Y, Fan L, Tuyishime P, Zheng P*, Sun J*. Synthetic Methylotrophy: A Practical Solution for Methanol-Based Biomanufacturing. Trends Biotechnol. 2020.

6.Zhang Z, Wang Y *, Zheng P* and Sun J.Promoting Lignin Valorization by Coping with Toxic C1 Byproducts. Trends Biotechnol. 2020.

7.Chen J #, Wang Y # , Guo X, Rao D, Zhou W, Zheng P*, Sun J * and Ma Y.Efficient bioproduction of 5？aminolevulinic acid, a promising biostimulant and nutrient, from renewable bioresources by engineered Corynebacterium glutamicum. Biotechnol Biofuels. 2020, 13(1)

8.Li M #, Chen J #, Wang Y, Liu J, Huang J, Chen N, Zheng P* and Sun J *. Efficient Multiplex Gene Repression by CRISPR-dCpf1 in Corynebacterium glutamicum. Front Bioeng Biotechnol. 2020, 8:357.

9.Zhang L #, Zheng X #, Cairns TC, Zhang Z, Wang D*, Zheng P* and Sun J. Disruption or reduced expression of the orotidine？5,？decarboxylase gene pyrG increases citric acid production: a new discovery during recyclable genome editing in Aspergillus niger. Microb Cell Fact. 2020, 19(1):1-12.

10.Dele-Osibanjo T#, Li Q #, Zhang X #, Guo X, Feng J, Liu J, Sun X, Wang X, Zhou W, Zheng P*, Sun J*, Ma Y. Growth-coupled evolution of phosphoketolase to improve l-glutamate production by Corynebacterium glutamicum. Appl Microb Biotech. 2019

11.Wang W#, Li Q#, Sun C, Cai Z, Zheng X, Guo X, Ni X, Zhou W, Guo Y, Zheng P*, Chen C, Sun J*, Li Y, Ma Y. GREACE-assisted adaptive laboratory evolution in endpoint fermentation broth enhances lysine production by Escherichia coli. Microb Cell Fact. 2019

12.Wang Y#, Liu Y#, Li J, Yang Y, Ni X, Cheng H, Huang H, Guo Y, Ma H, Zheng P*, Wang M*, Sun J, Ma Y. Expanding targeting scope, editing window, and base transition capability of base editing in Corynebacterium glutamicum. Biotech Bioeng. 2019

13.Zhu C#, Chen J#, Wang Y, Wang L, Guo X, Chen N, Zheng P*, Sun J*, Ma Y. Enhancing 5‐aminolevulinic acid tolerance and production by engineering the antioxidant defense system of Escherichia coli. Biotechnol Bioeng. 2019

14.Sun D#, Chen J#, Wang Y, Li M, Rao D, Guo Y, Chen N, Zheng P*, Sun J*, Ma Y. Metabolic Engineering of Corynebacterium glutamicum by Synthetic Small Regulatory RNAs. J Industr Microb & Biotech, 2019, 46 (2): 203–208.

15.Tuyishime P#, Wang Y#, Fan L, Zhang Q, Li Q, Zheng P*, Sun J*, Ma Y. Engineering Corynebacterium glutamicum for methanol-dependent growth and glutamate production. Metab Eng, 2018, 49:20-231.

16.Wang Y#, Liu Y #, Liu J, Guo Y, Fan L, Ni X, Zheng X, Wang M*, Zheng P*, Sun J*, Ma Y. MACBETH: Multiplex automated Corynebacterium glutamicum base editing method. Metab Eng, 2018, 47: 200-210.

17.Zheng X, Zheng P, Zhang K, Cairns TC, Meyer V, Sun J*, Ma Y. 5S rRNA Promoter for Guide RNA Expression Enabled Highly Efficient CRISPR/Cas9 Genome Editing in Aspergillus niger. ACS Synth Biol., 2018, e7b00456.

18.Zhang Q#, Zheng X#, Wang Y, Yu J, Zhang Z, Dele-Osibanjo T, Zheng P*, Sun J*, Jia S, Ma Y. Comprehensive Optimization of the Metabolomics Methodology for Metabolite Profiling of Corynebacterium glutamicum. Appl Microb Biotech, 2018, 102 (16): 7113-7121.

19.Wang Y, Cao G, Xu D, Fan L, Wu X, Ni X, Zhao S, Zheng P*, Sun J*, Ma Y. A Novel L-Glutamate Exporter of Corynebacterium glutamicum. Appl Enviro Microb, 2018, 84(6): e02691-17