The major interests of the lab is fungal synthetics biology, including (1) developing the enabling techniques of fungal synthetic biology, including genome editing tools, metabolic engineering techniques and so on; (2) developing the heterologous protein expression system in filamentous fungi for production of industrial enzymes and other proteins; (3) construction of fungal cell factory for bulk chemicals production from lignocellulose, including organic acid, bioethanol and so on.
1993.9-1997.7 Bachelor student, Department of Biology, Beijing Normal University
1997.9-2000.7 Master student, Institute of Subtropical Forestry, Chinese Academy of Forestry
2000.9-2004.12 Ph.D student, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
2005.1-2009.12 Postdoc, Department of Plant and Microbial Biology, University of California at Berkeley
2009.12-now PI, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
2021-2022 Director, System Microbial Engineering Lab of CAS
2022-now Deputy director, Key Laboratory of Engineering Biology for Low-carbon Manufacturing
1. Liu D, Xu Z, Li J, Liu Q, Yuan Q, Guo Y, Ma H, Tian C: Reconstruction and analysis of genome-scale metabolic model for thermophilic fungus Myceliophthora thermophila. Biotechnol Bioeng 2022.
2. Wang H, Sun T, Zhao Z, Gu S, Liu Q, Wu T, Wang D, Tian C, Li J: Transcriptional Profiling of Myceliophthora thermophila on Galactose and Metabolic Engineering for Improved Galactose Utilization. Front Microbiol 2021, 12:664011.
3. Li J, Chen B, Gu S, Zhao Z, Liu Q, Sun T, Zhang Y, Wu T, Liu D, Sun W et al: Coordination of consolidated bioprocessing technology and carbon dioxide fixation to produce malic acid directly from plant biomass in Myceliophthora thermophila. Biotechnol Biofuels 2021, 14(1):186.
4. Li J, Liu Q, Li J, Lin L, Li X, Zhang Y, Tian C: RCO-3 and COL-26 form an external-to-internal module that regulates the dual-affinity glucose transport system in Neurospora crassa. Biotechnol Biofuels 2021, 14(1):33.
5. Li X, Liu Q, Sun W, He Q, Tian C: Improving cellulases production by Myceliophthora thermophila through disruption of protease genes. Biotechnol Lett 2020, 42(2):219-229.
6. Li J, Zhang Y, Li J, Sun T, Tian C: Metabolic engineering of the cellulolytic thermophilic fungus Myceliophthora thermophila to produce ethanol from cellobiose. Biotechnol Biofuels 2020, 13:23.
7. Li J, Lin L, Sun T, Xu J, Ji J, Liu Q, Tian C: Direct production of commodity chemicals from lignocellulose using Myceliophthora thermophila. Metab Eng 2020, 61:416-426.
8. Li F, Liu Q, Li X, Zhang C, Li J, Sun W, Liu D, Xiao D, Tian C: Construction of a new thermophilic fungus Myceliophthora thermophila platform for enzyme production using a versatile 2A peptide strategy combined with efficient CRISPR-Cas9 system. Biotechnol Lett 2020, 42(7):1181-1191.
9. Liu Q, Zhang Y, Li F, Li J, Sun W, Tian C: Upgrading of efficient and scalable CRISPR-Cas-mediated technology for genetic engineering in thermophilic fungus Myceliophthora thermophila. Biotechnol Biofuels 2019, 12:293.
10. Li J, Gu S, Zhao Z, Chen B, Liu Q, Sun T, Sun W, Tian C: Dissecting cellobiose metabolic pathway and its application in biorefinery through consolidated bioprocessing in Myceliophthora thermophila. Fungal Biol Biotechnol 2019, 6:21.
11. Lin L, Wang S, Li X, He Q, Benz JP, Tian C: STK-12 acts as a transcriptional brake to control the expression of cellulase-encoding genes in Neurospora crassa. PLoS Genet 2019, 15(11):e1008510.
12. Xu G, Li J, Liu Q, Sun W, Jiang M, Tian C: Transcriptional analysis of Myceliophthora thermophila on soluble starch and role of regulator AmyR on polysaccharide degradation. Bioresour Technol 2018, 265:558-562.
13. Gu S, Li J, Chen B, Sun T, Liu Q, Xiao D, Tian C: Metabolic engineering of the thermophilic filamentous fungus Myceliophthora thermophila to produce fumaric acid. Biotechnol Biofuels 2018, 11:323.
14. Lin L, Sun Z, Li J, Chen Y, Liu Q, Sun W, Tian C: Disruption of gul-1 decreased the culture viscosity and improved protein secretion in the filamentous fungus Neurospora crassa. Microb Cell Fact 2018, 17(1):96.
15. Liu Q, Gao R, Li J, Lin L, Zhao J, Sun W, Tian C: Development of a genome-editing CRISPR/Cas9 system in thermophilic fungal Myceliophthora species and its application to hyper-cellulase production strain engineering. Biotechnol Biofuels 2017, 10:1.
16. Wang B, Li J, Gao J, Cai P, Han X, Tian C: Identification and characterization of the glucose dual-affinity transport system in Neurospora crassa: pleiotropic roles in nutrient transport, signaling, and carbon catabolite repression. Biotechnol Biofuels 2017, 10:17.
17. Lin L, Chen Y, Li J, Wang S, Sun W, Tian C: Disruption of non-anchored cell wall protein NCW-1 promotes cellulase production by increasing cellobiose uptake in Neurospora crassa. Biotechnol Lett 2017, 39(4):545-551.
18. Pei X, Zhao J, Cai P, Sun W, Ren J, Wu Q, Zhang S, Tian C: Heterologous expression of a GH3 beta-glucosidase from Neurospora crassa in Pichia pastoris with high purity and its application in the hydrolysis of soybean isoflavone glycosides. Protein Expr Purif 2016, 119:75-84.
19. Xu J, Li J, Lin L, Liu Q, Sun W, Huang B, Tian C: Development of genetic tools for Myceliophthora thermophila. BMC Biotechnol 2015, 15:35.
20. Pei X, Fan F, Lin L, Chen Y, Sun W, Zhang S, Tian C: Involvement of the adaptor protein 3 complex in lignocellulase secretion in Neurospora crassa revealed by comparative genomic screening. Biotechnol Biofuels 2015, 8:124.
Over 10 projects involved in fungal synthetic biology are undergoing supported by National Natural Science Foundation of China, National Key R&D Program from Ministry of Science and Technology of China, Tianjin Municipal Government and the biological related Companies.
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