Title:Zhang Xueli
Fax:022-84861983
E-mail:zhang_xl@tib.cas.c
Our group's research interests are Synthetic Biology and Gene Editing. We focus on (1) constructing microbial cell factories for production of amino acids, vitamins, material chemicals and plant-derived natural products through metabolic engineering and synthetic biology; (2) developing new gene editing technologies and their application in gene therapy of genetic diseases.
1996.09-2000.07 Shanghai Jiao Tong University, Bachelor
2000.09-2005.07 Shanghai Jiao Tong University, Ph.D
2005.08-2007.07 University of Florida, Postdoc
2007.07-2010.01 University of Florida, Research Assistant Professor
2010.01-present Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Principal Investigator
Xueli Zhang was supported by the Outstanding Youth Program and the Excellent Youth Program of National Natural Science Foundation of China.
He was awarded the first prize for Technical Invention from China National Light Industry Council and the China Excellence Patent Award.
1. Zhao D, Li J, Li S, Xin X, Hu M, Marcus A. Price, Susan J. Rosser, Bi C*, Zhang X*. Glycosylase base editors enable C-to-A and C-to-G base changes. Nature Biotechnology. 2021, 39:35–40.
2. Wu Y, Wan X, Zhao D, Chen X, Wang J, Tang X, Li J, Li S, Sun X*, Bi C*, Zhang X*. AAV-mediated base-editing therapy ameliorates the disease phenotypes in a mouse model of retinitis pigmentosa. Nature Communications. 2023, 14(1):4923.
3. Yang C, Ma Z, Wang K, Dong X, Huang M, Li Y, Zhu X, Li J, Cheng Z, Bi C*, Zhang X*. HMGN1 enhances CRISPR-directed dual-function A-to-G and C-to-G base editing. Nature Communications. 2023, 14(1):2430.
4. Chen R, Cao Y, Liu Y, Zhao D, Li J, Cheng Z, Bi C*, Zhang X*. Enhancement of a prime editing system via optimal recruitment of the pioneer transcription factor. Nature Communications. 2023, 14(1):257.
5. Li S, An J, Li Y, Zhu X, Zhao D, Wang L, Sun Y, Yang Y, Bi C*, Zhang X*, Wang M*. Automated high-throughput genome editing platform with an AI learning in situ prediction model. Nature Communications. 2022, 13(1):7386.
6. Zhu H, Xu L, Luan G, Zhan T, Kang Z, Li C, Lu X*, Zhang X*, Zhu Z*, Zhang Y*, Li Y*. A miniaturized bionic ocean-battery mimicking the structure of marine microbial ecosystems. Nature Communications. 2022, 13(1):5608.
7. Zhao D, Jiang G, Li J, Chen X, Li S, Wang J, Zhou Z, Pu S, Dai Z, Ma Y, Bi C*, Zhang X*. Imperfect guide-RNA (igRNA) enables CRISPR single-base editing with ABE and CBE. Nucleic Acids Research. 2022, 50:4161-4170.
8. Wang P, Zhao D, Li J, Su J, Zhang C, Li S, Fan F, Dai Z, Liao X, Mao Z, Bi C*, Zhang X*. Artificial diploid Escherichia coli by a CRISPR chromosome-doubling technique. Advanced Science. 2023, 10(7):e2205855.
9. Yang C, Dong X, Ma Z, Li B, Bi C*, Zhang X*. Pioneer factor improves CRISPR-based C-to-G and C-to-T Base Editing. Advanced Science. 2022, 9(26):e2202957.
10. Xi Y, Xu H, Zhan T, Qin Y, Fan F*, Zhang X*. Metabolic engineering of the acid-tolerant yeast Pichia kudriavzevii for efficient L-malic acid production at low pH. Metab Eng. 2022, 75:170-180.
11. Xu L, Wang D, Chen J, Li B, Li Q, Liu P, Qin Y, Dai Z*, Fan F*, Zhang X*. Metabolic engineering of Saccharomyces cerevisiae for gram-scale diosgenin production. Metab Eng. 2022, 70:115-128.
12. Wang J, Zhao D, Li J, Hu M, Xin X, Price MA, Li Q, Liu L, Li S, Rosser SJ, Zhang C*, Bi C*, Zhang X*. Helicase-AID. A novel molecular device for base editing at random genomic loci. Metab Eng. 2021, 67:396-402.
13. Shi Y, Wang D, Li R, Huang L, Dai Z*, Zhang X*. Engineering yeast subcellular compartments for increased production of the lipophilic natural products ginsenosides. Metab Eng. 2021, 67:104-111.
14. Zhao D, Zhu X, Zhou H, Sun N, Wang T, Bi C*, Zhang X*. CRISPR-based metabolic pathway engineering. Metab Eng. 2021, 63:148-159.
15. Wang D, Wang J, Shi Y, Li R, Fan F, Huang Y, Li W, Chen N, Huang L, Dai Z*, Zhang X*. Elucidation of the complete biosynthetic pathway of the main triterpene glycosylation products of Panax notoginseng using a synthetic biology platform. Metab Eng. 2020, 61:131-140.
16. Yu Y, Shao M, Li D, Fan F, Xu H, Lu F, Bi C, Zhu X*, Zhang X*. Construction of a carbon-conserving pathway for glycolate production by synergetic utilization of acetate and glucose in Escherichia coli. Metab Eng. 2020, 61:152-159.
17. Chen J, Fan F, Qu G, Tang J, Xi Y, Bi C, Sun Z*, Zhang X*. Identification of Absidia orchidis steroid 11β-hydroxylation system and its application in engineering Saccharomyces cerevisiae for one-step biotransformation to produce hydrocortisone. Metab Eng. 2020, 57:31-42.
18. Yu Y, Zhu X, Xu H, Zhang X*. Construction of an energy-conserving glycerol utilization pathways for improving anaerobic succinate production in Escherichia coli. Metab Eng. 2019, 56:181-189.
19. Dai Z, Liu Y, Sun Z, Wang D, Qu G, Ma X, Fan F, Zhang L, Li S, Zhang X*. Identification of a novel cytochrome P450 enzyme that catalyzes the C-2α hydroxylation of pentacyclic triterpenoids and its application in yeast cell factories. Metab Eng. 2019, 51:70-78.
20. Li Q, Fan F, Gao X, Yang C, Bi C, Tang J, Liu T, Zhang X*. Balanced activation of IspG and IspH to eliminate MEP intermediate accumulation and improve isoprenoids production in Escherichia coli. Metab Eng. 2017, 44:13-21.
21. Wu T, Ye L, Zhao D, Li S, Li Q, Zhang B, Bi C*, Zhang X*. Membrane engineering - A novel strategy to enhance the production and accumulation of β-carotene in Escherichia coli. Metab Eng. 2017, 43:85-91.
22. Zhu X, Zhao D, Qiu H, Fan F, Man S, Bi C*, Zhang X*. The CRISPR/Cas9-facilitated multiplex pathway optimization (CFPO) technique and its application to improve the Escherichia coli xylose utilization pathway. Metab Eng. 2017, 43:37-45.
23. Zhu X, Tan Z, Xu H, Chen J, Tang J, Zhang X*. Metabolic evolution of two reducing equivalent-conserving pathways for high-yield succinate production in Escherichia coli. Metab Eng. 2014, 24:87-96.
24. Dai Z, Liu Y, Zhang X, Shi M, Wang B, Wang D, Huang L*, Zhang X*. Metabolic engineering of Saccharomyces cerevisiae for production of ginsenosides. Metab Eng. 2013, 20:146-156.
25. Zhao J, Li Q, Sun T, Zhu X, Xu H, Tang J, Zhang X*, Ma Y. Engineering central metabolic modules of Escherichia coli for improving β-carotene production. Metab Eng. 2013, 17: 42-50.
26. Shi A, Zhu X, Lu J, Zhang X*, Ma Y. Activating transhydrogenase and NAD kinase in combination for improving isobutanol production. Metab Eng. 2013, 16:1-10.
1、The Outstanding Youth Program of National Natural Science Foundation of China, Microbial Cell Factory (32225031). 4 million yuan. Project leader. 2023.01- 2027.12.
2、The National Key Research and Development Program of China, The Establishment and Utilization of Microbial Chemical Factories (2019YFA0904900). 21.08 million. Project leader. 2020.01-2024.12.
3、The Excellent Youth Program of National Natural Science Foundation of China, Microbial Genetics and Breeding (31522002). 1.5 million yuan. Project leader. 2016.01-2018.12.
4、The National High Technology Research and Development Program of China (863 Program), Genome Scale Systems Metabolic Engineering.（2012AA022104）. 4.84 million yuan. Project leader. 2012.11-2015.12.
5、The Key Research Program of Chinese Academy of Sciences, Synthesis of non-natural yeast Genome for productions of Plant-derived Natural Products. (KFZD-SW-215). 15 million yuan. Project leader. 2018.01-2019. 12.
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