Large scale absolute protein quantitation for industrial cell factory.

2.  Bacterial protein post translational modification (PTMs) identification.

3. Protein-centered biological molecular interact network identification (crosslinking-MS based, proximity labeling based, et al).

Qichen Cao, Ph.D., Associate researcher, Systems Biology Centre, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences. From 2007 to 2014, he worked as phase I clinical trial pharmacokinetic researcher at the National Drug Clinical Trial Institute of the Affiliated Hospital of Liaoning University of Traditional Chinese Medicine. He mainly conducted the detection of clinical drugs and their metabolites from human plasma, based on the MRM/SRM technology of mass spectrometry. From 2011 to 2014, he studied at Beijing Proteomics Research Center for his Ph.D. He worked for the projects of "Large-scale identification of core-fucosylated proteome" and "Large-scale identification of 2-Hydroxyisobutyrylation from Saccharomyces cerevisiae". He joined Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences in 2014, primarily engaging in mass spectrometry-based industrial microbial systems biology research. Applying proteomics to elucidate the life mechanisms of industrial strains and fermentation processes. To date, he has published over ten research papers in journals such as Analytical Chemistry and Journal of Proteomics Research. He also has been granted 2 (Chinese) invention patents.

1. Science and Technology Progress Award of Liaoning Province （Second prize, 2010）

2. Science and Technology Progress Award of Liaoning Province （Third prize, 2007）

1. Cao, Q.; Han, M.; Zhang, Z.; Yu, C.; Xu, L.; Shi, T.; Zheng, P.; Sun, J. Novel 15N Metabolic Labeling-Based Large-Scale Absolute Quantitative Proteomics Method for Corynebacterium Glutamicum. Anal. Chem. 2023, 95 (11), 4829–4833.

2. Sun, X.; Mao, Y.; Luo, J.; Liu, P.; Jiang, M.; He, G.; Zhang, Z.; Cao, Q.; Shen, J.; Ma, H.; Chen, T.; Wang, Z. Global Cellular Metabolic Rewiring Adapts Corynebacterium Glutamicum to Efficient Nonnatural Xylose Utilization. Appl. Environ. Microbiol. 2022, 88 (23), e0151822.

3. Xiong, Y.; Guo, Y.; Xiao, W.; Cao, Q.; Li, S.; Qi, X.; Zhang, Z.; Wang, Q.; Shui, W. An NGS-Independent Strategy for Proteome-Wide Identification of Single Amino Acid Polymorphisms by Mass Spectrometry. Anal. Chem. 2016, 88 (5), 2784–2791.

4. Xiao, W.; Duan, X.; Lin, Y.; Cao, Q.; Li, S.; Guo, Y.; Gan, Y.; Qi, X.; Zhou, Y.; Guo, L.; Qin, P.; Wang, Q.; Shui, W. Distinct Proteome Remodeling of Industrial Saccharomyces Cerevisiae in Response to Prolonged Thermal Stress or Transient Heat Shock. J. Proteome Res. 2018, 17 (5), 1812–1825.

5. Miao, C.; Yang, Y.; Li, S.; Guo, Y.; Shui, W.; Cao, Q. Discrimination and Quantification of Homologous Keratins from Goat and Sheep with Dual Protease Digestion and PRM Assays. J. Proteomics 2018, 186, 38–46.

6. Li, Z.; Li, Y.; Chen, W.; Cao, Q.; Guo, Y.; Wan, N.; Jiang, X.; Tang, Y. J.; Wang, Q.; Shui, W. Integrating MS1 and MS2 Scans in High-Resolution Parallel Reaction Monitoring Assays for Targeted Metabolite Quantification and Dynamic (13)C-Labeling  Metabolism Analysis. Anal. Chem. 2017, 89 (1), 877–885.

7. Li, X.; Jiang, J.; Zhao, X.; Zhao, Y.; Cao, Q.; Zhao, Q.; Han, H.; Wang, J.; Yu, Z.; Peng, B.; Ying, W.; Qian, X. In-Depth Analysis of Secretome and N-Glycosecretome of Human Hepatocellular Carcinoma Metastatic Cell Lines Shed Light on Metastasis Correlated Proteins. Oncotarget 2016, 7 (16), 22031–22049.

8. Li, S.; Cao, Q.; Xiao, W.; Guo, Y.; Yang, Y.; Duan, X.; Shui, W. Optimization of Acquisition and Data-Processing Parameters for Improved Proteomic Quantification by Sequential Window Acquisition of All Theoretical Fragment Ion Mass Spectrometry. J. Proteome Res. 2017, 16 (2), 738–747.

9. Huang, J.; Luo, Z.; Ying, W.; Cao, Q.; Huang, H.; Dong, J.; Wu, Q.; Zhao, Y.; Qian, X.; Dai, J. 2-Hydroxyisobutyrylation on Histone H4K8 Is Regulated by Glucose Homeostasis in Saccharomyces Cerevisiae. Proc. Natl. Acad. Sci. U. S. A. 2017, 114 (33), 8782–8787.

10. Cao, Q.; Zhao, X.; Zhao, Q.; Lv, X.; Ma, C.; Li, X.; Zhao, Y.; Peng, B.; Ying, W.; Qian, X. Strategy Integrating Stepped Fragmentation and Glycan Diagnostic Ion-Based Spectrum Refinement for the Identification of Core Fucosylated Glycoproteome  Using Mass Spectrometry. Anal. Chem. 2014, 86 (14), 6804–6811.

11. Cao, Q.; Zhao, Q.; Qian, X.; Ying, W. Identification of Core-Fucosylated Glycoproteome in Human Plasma. Methods Mol. Biol. Clifton NJ 2017, 1619, 127–137.

12. Cao, Q.; Ma, C.; Bai, H.; Li, X.; Yan, H.; Zhao, Y.; Ying, W.; Qian, X. Multivalent Hydrazide-Functionalized Magnetic Nanoparticles for Glycopeptide Enrichment and Identification. The Analyst 2014, 139 (3), 603–609.

13.Wang, W.; Cao, Q.; Gao, J.; Wang, D.; Pharmacokinetic studies about Herbaceous compatible sense of Zuojinwan. Chinese Journal of Clinical Pharmacology. 2010, 26(2):126-128

14. Wang Wen.; Cao Q.; Wang H.; LV Y.; Pharmacokinetic study on different compatibilities of Dangguibuxue Tang. Chinese Journal of Clinical Pharmacology. 2009, 14(6):659-663

1. National Natural Science Foundation of China，Youth Fund Project (No. 21505151)

2. Subtask of National Key R&D Program of China (2018YFA0901501)