Associate Professor/Senior Engineer
Dong Huina

Title:Dong Huina

Tel:0086-22-84861945

E-mail:dong_hn@tib.cas.cn

Research Interest

Vitamin Biosynthesis, Microbial Cell Factory, Microbial metabolic Engineering, Synthetic Biotechnology

Education & Professional Experience

2005-2009  Hebei University of Technology, Tianjin, PR China, B.S.

2009-2012  Tianjin University, Tianjin, PR China, M.S.

2012-2016 Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Research intern

2016-2021 Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Research assistant

2021-至今Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences,Associate Investigator

Honors and Awards

 "the First Class" of Tianjin Science and Technology Progress Award in 2020 (Rank Second)

"the Second Class" of Science and Technology Progress Award by China National Light Industry Council in 2020 (Rank Second)

Selected Publications

1. Guangqing Liu#Huiying Wang#Baisong Tong#Yali CuiSibylle C VoneschHuina Dong*, Dawei Zhang*, An Efficient CRISPR/Cas12e System for Genome Editing in Sinorhizobium meliloti, ACS Synth Biology. 2023 Mar 17;12(3):898-903. 

2. Yali Cui#Huina Dong#Baisong TongHuiying WangXipeng ChenGuangqing Liu, Dawei Zhang*, A versatile Cas12k-based genetic engineering toolkit (C12KGET) for metabolic engineering in genetic manipulation-deprived strains, Nucleic Acids Research. 2022 Aug 26;50(15):8961-8973. doi: 10.1093/nar/gkac655.

3. Huina Dong#, Yali Cui#, Dawei Zhang*, CRISPR/Cas Technologies and Their Applications in Escherichia coli,Frontiers in Bioengineering and Biotechnology, 11 November 2021, 9, 1-10.

4. Zhenquan Liu#Huina Dong#Yali Cui#Lina Cong*, Dawei Zhang*, Application of different types of CRISPR/Cas-based systems in bacteria, Microbial Cell Factories. 2020 Sep 3;19(1):172.

5. Zhenquan Liu#Huina Dong#Xiangying WuHuan FangLina Cong*, Beiwei Zhu, Dawei Zhang*, Identification of a xylose-inducible promoter and its application for improving vitamin B12 production in Sinorhizobium meliloti, Biotechnology and Applied Biochemistry. 2021 Aug;68(4):856-864.

6. Yali Cui #Huina Dong#, Yuanyuan Ma*, and Dawei Zhang*.Strategies for Applying Nonhomologous End Joining-Mediated Genome Editing in Prokaryotes. ACS Synthetic Biology. 2019 Oct 18;8(10):2194-2202.

7. Huina Dong#, Sha Li#, Huan Fang, Miaomiao Xia, Ping Zheng*, Dawei Zhang*, Jibin Sun. A newly isolated and identified vitamin B12 producing strain: Sinorhizobium meliloti 320. Bioprocess and Biosystems Engineering. 2016 Oct;39(10):1527-37.

8. Huina Dong, Yongfei Liu, Xin Zu, Ning Li, Feiran Li, Dawei Zhang*.An enzymatic assay for high-throughput screening of cytidine-producing microbial strains. PloS One.  2015 Mar 27; 10 (3):e0121612.

9. Huina Dong#, Xin Zu#, Ping Zheng, Dawei Zhang*. A rapid enzymatic assay for high-throughput screening of adenosine-producing strains. Microbial Biotechnology. 2015 Mar; 8(2):230-8. 

Project

National Key R&D Program of China

National Natural Science Foundation of China

Natural Science Foundation of Tianjin City

Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project