1. Establishment and optimization of protein expression system in Bacillus subtilis and efficient expression of various high-value protein products

2. Biosynthesis technology of vitamins B and K

2010.07- 2012.08 Tianjin Institute of Pharmaceutical Research, Drug Innovation Research Centre, Research Intern

2012.08- 2014.04 Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Research Intern

2014.04- 2018.02 Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Assistant Researcher III

2018.02- 2020.07 Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Assistant Researcher I

2020.07- Present Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Associate Researcher III

1. Jinlong Li, Sijia Wang, Cui Liu, Yixin Li, Yu Wei, Gang Fu, Pi Liu, Hongwu Ma, Dawei Huang, Jianping Lin* and Dawei Zhang*. Going beyond the local catalytic activity space of chitinase using a simulation-based iterative saturation mutagenesis strategy. ACS Catalysis. 2022 Aug 5. 12, 16, 10235–10244

2. Fu, G.; Yue, J.; Li, D.; Li, Y.; Lee, S. Y.; Zhang, D., An operator-based expression toolkit for Bacillus subtilis enables fine-tuning of gene expression and biosynthetic pathway regulation. Proc Natl Acad Sci U S A 2022, 119 (11), e2119980119.

3. Yuan, M.; Fu, G.; Sun, Y. M.; Zhang, D. W., Biosynthesis and applications of curdlan. Carbohyd Polym 2021, 273.

4. Zhao, L. Q.; Cui, Y. Y.; Fu, G.; Xu, Z. X.; Liao, X. P.; Zhang, D. W., Signal Recognition Particle Suppressor Screening Reveals the Regulation of Membrane Protein Targeting by the Translation Rate. Mbio 2021, 12 (1).

5. Wang, S.; Fu, G.; Li, J.; Wei, X.; Fang, H.; Huang, D.; Lin, J.; Zhang, D., High-Efficiency Secretion and Directed Evolution of Chitinase BcChiA1 in Bacillus subtilis for the Conversion of Chitinaceous Wastes into Chitooligosaccharides. Front Bioeng Biotechnol 2020, 8, 432.

6. Gang Fu, Shibin Zhang, Huina Dong, Jingqi Chen, Ran Tu, Dawei Zhang, Enhanced production of d-psicose 3-epimerase in Bacillus subtilis by regulation of segmented fermentation. Biotechnol Appl Biochem. 2020 Sep;67(5):812-818.

7. Li, D.#; Fu, G.#; Tu, R.; Jin, Z.; Zhang, D., High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components. Microbial cell factories 2019, 18 (1), 17.

8. Fu, G.; Liu, J.; Li, J.; Zhu, B. W.; Zhang, D., Systematic screening of optimal signal peptides for secretory production of heterologous proteins in Bacillus subtilis. Journal of agricultural and food chemistry 2018, 66 (50), 13141-13151.

1. High-efficiency synthesis of menaquinone-7 technology in cell factories, Tianjin Synthetic Biotechnology Innovation Capacity Enhancement Action, 2023.11-2024.10, 1 million, project leader

2. Research on localization of substitutes for imported core raw materials for 'reproductive health series testing products', Tianjin Synthetic Biotechnology Innovation Capacity Enhancement Action, 2021.03-2024.02, 480,000, Project Leader;

3. Mechanism of signal peptide amino acid composition on secretion efficiency in Bacillus licheniformis, National Natural Science Foundation of China Emergency Management Program, 2015.01-2015.12, 100,000, project leader

4. Analysis of the regulatory mechanism of the maltose transcription factor glvR in Bacillus subtilis and the application of its expression system, National Natural Science Foundation of China (NSFC) Youth Fund Project, 2016.01-2018.12, 210,000, Project Leader

5.Research on mining new pathway of protein transport using ΔSRP repressor, National Natural Science Foundation of China General Program, 2014.01-2017.12, 840,000, core member

6. High-level secreted producing of recombinant human FGF21 protein，NN-CAS Research Fund，2016.02-2017.12, 1.2 million, core member

7. Biosynthesis and scale-up production of lysozyme, Tianjin Programs for Science and Technology Development, 750,000, core member