1. Development of efficient genome-editing tools and superior elements for fine-tuning gene expression in Corynebacterium glutamicum, which is an important industrial chassis for amino acid production.

2. Mining and engineering functional elements for biosynthesis of amino acids.

3. Designing and engineering the industrial strains for amino acid production.

2002-09 to 2006-07, B.Sc. in Bioengineering, Hunan Agricultural University, China

2006-09 to 2009-06, M.Sc. in Biochemistry and Molecular Biology, Guanxi University, China

2009-07 to 2014-04, Research assistant, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China

2014-04 to 2017-12, Assistant professor, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China

2017-12 to 2020-07, Engineer, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China

2020-07 to present, Senior engineer, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China

2020, First Prize of Scientific and Technological Progress in Inner Mongolia

2019, CAS Science and Technology Promotion and Development Prize

2014, China Industry-University-Research Cooperation Innovation Achievement Award

1. Liu J, Liu M, Shi T, Sun G, Gao N, Zhao X, Guo X, Ni X, Yuan Q, Feng J, et al. CRISPR-assisted rational flux-tuning and arrayed CRISPRi screening of an l-proline exporter for l-proline hyperproduction. Nature Communications. 2022;13:891.

2. Liu J, Wang Y, Lu Y, Ni X, Guo X, Zhao J, Chen J, Dele-Osibanjo T, Zheng P, Sun J, Ma Y. Mutations in Peptidoglycan Synthesis Gene ponA Improve Electrotransformation Efficiency of Corynebacterium glutamicum ATCC 13869. Appl Environ Microbiol. 2018;84.

3. Liu J, Wang Y, Zheng P, Sun J. CRISPR/Cas9-mediated ssDNA Recombineering in Corynebacterium glutamicum. Bio Protoc. 2018;8:e3038.

4. Liu J, Wang Y, Lu Y, Zheng P, Sun J, Ma Y. Development of a CRISPR/Cas9 genome editing toolbox for Corynebacterium glutamicum. Microb Cell Fact. 2017;16:205.

5. Wang XC, Liu J, Zhao J, Ni XM, Zheng P, Guo X, Sun CM, Sun JB, Ma YH. Efficient production of trans-4-hydroxy-l-proline from glucose using a new trans-proline 4-hydroxylase in Escherichia coli. J Biosci Bioeng. 2018;126:470-477.

6. Hu X, Huang X, Liu J, Zheng P, Gong W, Yang L. Structures of L-proline trans-hydroxylase reveal the catalytic specificity and provide deeper insight into AKG-dependent hydroxylation. Acta Crystallogr D Struct Biol. 2023;79:318-325.

7. Wang Y, Liu Y, Liu J, Guo Y, Fan L, Ni X, Zheng X, Wang M, Zheng P, Sun J, Ma Y. MACBETH: Multiplex automated Corynebacterium glutamicum base editing method. Metab Eng. 2018;47:200-210.

1. National Key R&D Program of China, Ministry of Science and Technology of PR China, System engineering the industrial strains of important amino acids and their industrial applications, 2021.07-2025.06

2. National Key R&D Program of China, Ministry of Science and Technology of PR China, Frontier technology for the bioengineering manufacture of protein feed and developing new products, 2021.12-2024.11

3. Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project, Tianjin Municipal Science and Technology Bureau, Iterative development of the industrial strains for amino acid production, 2019.10-2022.09

4. National Natural Science Foundation of China, Identification of L-proline exporter in Corynebacterium glutamicum and its functional studies, 2019.01-2021.12

5. Key Deployment Project of Chinese Academy of Sciences, Biosynthesis of apis and drug intermediates, 2017.05-2019.04