I have been engaged in long-term research in metabolic engineering and systems biology, and have successfully constructed microbial cell factories/in vitro enzymatic catalysis systems for the high-yield production of various platform compounds (such as acetoin, succinic acid, 2,3-butanediol, etc.). I have designed and validated novel non-natural pathways for the assimilation of one-carbon compounds and the synthesis of chemicals.

In recent years, my focus has been on the automation of protein/ strain construction based on TIB biofoundry. We have developed the first automated high-throughput genome editing and design cloud platform (AutoESD). This platform supports fully automated design for gene editing tasks across species, technologies, and genomic loci, allowing for any type of modification. It can simultaneously process hundreds of design tasks with thousands of design targets, providing a significant boost to the automation level and practical editing throughput in just a few minutes.

1、Education Background

09/2007 to 06/2011, Tianjin University, Bachelor in Bioengineering.

09/2011 to 06/2018, Tianjin University, Ph.D. in Biochemical Engineering.

2、Work Experience

07/2018 to 07/2022, Postdoctoral Researcher at Laboratory of Biological Design Center, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences.

07/2022 to Present, Associate Researcher (Master Student Supervisor) at Laboratory of High-Throughput Editing and Screening Platform, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences.

Published 36 articles, cited 613 times, with an h-index of 15. Published 11 SCI research papers as the first or corresponding author, including 5 papers in the first quartile or Top journals. Applied for 15 invention patents, granted 6 patents, and registered 3 software copyrights.

Five Selected Publication:

[1]Yi Yang, Yufeng Mao (co_first) et al. AutoESD: A web tool for Automatic Editing Sequence Design for genetic manipulation of microorganisms. Nucleic Acids Research, 2022, 50(W1): W75-W82.

[2]Xi Sun, Yufeng Mao (co_first) et al. Global cellular metabolic rewiring adapts Corynebacterium glutamicum to Efficient non-natural xylose utilization. Applied and Environmental Microbiology, 2022, 16: e01518-01522.

[3]Yufeng Mao et al. Non-natural aldol reactions enable the design and construction of novel one-carbon assimilation pathways in vitro. Frontiers in Microbiology, 2021, 12(1360): 677596.

[4]Yufeng Mao et al. Metabolic engineering of Corynebacterium glutamicum for efficient production of succinate from lignocellulosic hydrolysate. Biotechnology for Biofuels, 2018, 11(1): 95.

[5]Yufeng Mao et al. Systematic metabolic engineering of Corynebacterium glutamicum for industrial-level production of optically pure D-(-)-Acetoin. Green Chemistry, 2017, 19(23): 5691-5702.

In recent years, I have led two sub-projects of the National Key Research and Development Program by the Ministry of Science and Technology, one project funded by the National Natural Science Foundation for Young Scientists, and one postdoctoral general project.