Currently, my primary focus lies in the fields of metabolic engineering and molecular pharmacognosy, as well as other innovative interdisciplinary research areas. These include the advancement and utilization of functional gene identification platforms, the development of efficient bionic synthetic cells, subcellular compartment engineering, the establishment of precise fermentation systems, and the amplification of production capacity. Notably, I have successfully generated a range of bionic synthetic cells utilizing Saccharomyces cerevisiae as the chassis microorganism, resulting in the creation of high-value natural products. One noteworthy achievement is the development of KH617, a Class I anticancer novel product line, which has demonstrated significant technological advancements.

1. Education Experience

2012.09-2015.03, Tianjin University of Science and Technology, major in light industry technology and engineering.

2. Professional Experience

2014.04-now, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, working as a research intern, assistant researcher, engineer, and senior engineer in turn. 

1. Liping Xu, Dong Wang, Jing chen, Bo Li, Qingyan Li, Pingping Liu, Ying Qin, Changhao Bi, Zhubo Dai*, Feiyu Fan*, Xueli Zhang*, Metabolic engineering of Saccharomyces cerevisiae for gram-scale diosgenin production, Metabolic Engineering, 2022, 70：115–128.

2. Rongsheng Li, Kou Wang, Dong Wang, Liping Xu, Yusong Shi, Zhubo Dai* and Xueli Zhang*, Production plant volatile terpenoids (Rose Oil) by yeast cell factories, Green Chemistry, 2021, 23: 5088-5096.

3. Weixian Li, Xiaohui Ma, Guodong Li, Aili Zhang, Dong Wang, Feiyu Fan, Xiaolin Ma, Xueli zhang*, Zhubo Dai* and Zigang Qian*, De novo biosynthesis of the oleanane-type triterpenoids of tunicosaponins in yeast, ACS Synthetic Biology, 2021, 10(8): 1874–1881.

4.  Yusong Shi, Dong Wang, Rongsheng Li, Luqi Huang, Zhubo Dai* and Xueli Zhang*, Engineering yeast subcellular compartments for increased production of the lipophilic natural products ginsenosides, Metabolic Engineering, 2021, 67: 104-111.

5. Dong Wang, Jinhe Wang, Yusong Shi, Rongsheng Li, Feiyu Fan, Yin Huang, Weixian Li, Ning Chen, Luqi Huang, Zhubo Dai* and Xueli Zhang*, Elucidation of the complete biosynthetic pathway of the main triterpene glycosylation products of Panax notoginseng using a synthetic biology platform, Metabolic Engineering, 2020, 61:131-140.

6. Zhubo Dai, Yun Liu, Zhoutong Sun, Dong Wang, Ge Qu, Lili Zhang, Shoulian Li, Xiaolin Ma, Feiyu Fan, Xueli Zhang*, Identification of a novel cytochrome P450 enzyme that catalyzes the C-2α hydroxylation of pentacyclic triterpenoids and its application in yeast cell factories, Metabolic Engineering, 2019,51:70-78.

7. Jiajian Huang, Wenlong Zha, Tianyue An，Yin Huang, Dong Wang,Tianyue An, Hua Dong, Rongmin Yu, Lixin Duan, Xueli Zhang, Reuben J. Peters*, Zhubo Dai*, Jiachen Zi*. Identification of RoCYP01 (CYP716A155) enables construction of engineered yeast for high-yield production of betulinic acid, Applied Microbiology and Biotechnology, 2019, 103(17):7029-7039.

I am presiding over two sub-projects of the National Key Research and Development Plan, and participated in one project each of the key deployment projects of the Chinese Academy of Sciences and the National Natural Science Foundation.