Title:Wei Hongli
E-mail:Wei_hl@tib.cas.cn
Senior engineer Wei Hongli is responsible for the management and service of Protein Preparation Module in Structural Biology Research Facility, she is familiar with the characteristics of various purification media and the use and maintenance of protein preparation equipment. Hongli Wei has been engaged in protein purification, structural analysis, protein characterization and modification for more than ten years, she has rich experiences in the establishment of protein preparation technologies, high-throughput preparation and structural analysis of proteins. With providing full-process services of protein preparation and protein structure analysis for lots of institutions and research groups, she has assisted in the preparation of hundreds of protein and resolved more than 30 protein structures and participated in publishing several SCI articles in Acs Catalysis, Green Chemistry and other journals, with the impact factor over 60 points.
(1) Expression, purification of proteins: Large-scale purification of soluble protein, inclusion body protein, anaerobic protein and membrane protein; High throughput preparation of proteins.
(2) Analysis of protein structures: Including glycosyltransferase, formaldehyde dehydrogenase, short-chain dehydrogenase, DNA regulatory protein, plastic degradation enzyme, terpene synthase, etc.
Education Background：
2003.09-2007.07，Beijing University of Chinese Medicine，Biopharmaceutical engineering，Bachelor's degree;
2007.09-2010.07，Shenyang Pharmaceutical university，Microbial and Biochemical Pharmacy，Master’s degree.
Working Experience：
2010.09-2011.09，Tianjin Meilun medicine group co., ltd，Drug development, Drug registration;
2011.10-2023.09，Tianjin institute of industrial biotechnology, Chinese academy of sciences，Research Assistant;
2023.09-Now，Tianjin institute of industrial biotechnology, Chinese academy of sciences，Senior Engineer.
（1）Li, T.#; Tang, Z.#; Wei, H.#; Tan, Z.; Liu, P.; Li, J.; Zheng, Y.; Lin, J.; Liu, W.; Jiang, H.; Liu, H*.; Zhu, L*.; Ma, Y*.; Totally atom-economical synthesis of lactic acid from formaldehyde: combined bio-carboligation and chemo-rearrangement without the isolation of intermediate, Green Chem. 2020, 22(9): 6809-6814.(共同一作）
（2）Zhang, T.#; Liu, P#; Wei, H; Sun, X.; Zeng, Y.; Zhang, X.; Cai, Y.; Cui, M.; Ma, H.; Liu, W.*; Sun, Y.*; Yang, J.*; Protein Engineering of Glucosylglycerol Phosphorylase Facilitating Efficient and Highly Regio- and Stereoselective Glycosylation of Polyols in a Synthetic System, ACS Catal. 2022, 12(24): 15715-15727.
（3）Janish, D.#; Liu, Y.; Wei, H.; Liu, W.; Ko, T.-P.; Guo, R.-T.; Eric, O.*; Structure, Function, and Inhibition of Staphylococcus aureus Heptaprenyl Diphosphate Synthase, ChemMedChem. 2016, 17(11): 1915-1923.
（4）Gerlis, V. H.; Han, X.; Lara, P.; Li, Q.; Wei, H.; Gao, J.; Karen, M.; Yufei, A.; Yannik, B.; Jan, M.; Christian, G. F.; Manfred, S. W.; David, B.; Gottfried, J. P.; Michael, L.; Michael, L.; Jiri, D.; Gert, W.; Liu, W; Uwe, T. B.; Wei, R.; Structural Insights into (Tere)phthalate-Ester Hydrolysis by a Carboxylesterase and Its Role in Promoting PET Depolymerization, ACS Catal. 2022, 12(24):15259-15270.
（5）Lee, S. M. Y.; Yip, T. F.; Sheng, Y.; Jin, D.Yan.; Wei, H.; Guo, R.-T.; Peiris, J. S. M.; Recognition of Double-Stranded RNA and Regulation of Interferon Pathway by Toll-Like Receptor 10, Front. Immunol. 2018, 9: 0-516.
（6）Li, Z.; Zhao, Y.; Wu, P.; Wang, H.; Li, Q.; Gao, J; Qin, H.-M.; Wei, H.; Uwe. T. B.; Han, X.; Wei, R.; Liu, W.; Structural insight and engineering of a plastic degrading hydrolase Ple629, Biochem. Biophys. Res. Commun. 2022,626:100-106.
（7）Zheng, Y.; Liu, W.; Chen, C.-C.; Hu, X.; Liu, W.; Ko, T.-P.; Tang, X.; Wei, H.; Huang, J. -W.; Guo, R.-T.; Crystal Structure of a Mycoestrogen-Detoxifying Lactonase from Rhinocladiella mackenziei: Molecular Insight into ZHD Substrate Selectivity, ACS Catal. 2018,8(5):4294-4298.
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