Monolith Microscale Thermophoresis System
Time:2024-12-19
- Obtain affinity (KD) and stoichiometric ratio of intermolecular interactions by measuring changes in biomolecular mobility in microscopic temperature gradient
- It takes only about 10min for determination of KD
- Equipped with two optical channels: UV autofluorescence and red fluorescence (590nm excitation and 660nm emission), non-fluorescent proteins can be quickly labeled with a labelling kit
- It can detect the interaction between proteins, nucleic acids, polysaccharides, liposomes, small molecules, ions and other molecules directly in solution, even in complex solutions such as cell lysate or serum, regardless of buffer composition
- Using high optical accuracy capillary loading, the overall dosage of fixed concentration molecule A is 200ul, and the total volume of stock solution molecule B is 20 μl, with concentration of about 20 * KD
- Low cost of consumables and easy operation