The High-Throughput Editing and Screening Laboratory of the Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, can provide a series of services. These include high-throughput automated preprocessing of samples, high-throughput automated gene editing, high-throughput automated protein purification and characterization, high-throughput automated metabolite detection, and high-throughput automated screening. These services offer strong technical support for the research and development in the field of synthetic biology. The main list of technical services provided is as follows:
|
Project Number |
Service Project |
Service Content |
|
1 |
Gene Cloning |
High-throughput gene cloning based on source DNA (e.g., bacterial genome) as needed |
|
2 |
Base Editing Plasmid Construction |
High-throughput construction of editing plasmids containing sgRNA |
|
3 |
Site-Directed |
Mutagenesis Plasmid Constructionor high-throughput construction of single or double point mutation plasmids |
|
4 |
Corynebacterium Glutamicum Genome Base Editing |
High-throughput base editing of the Corynebacterium glutamicum genome using base editing plasmids |
|
5 |
Bacillus Subtilis Genome Base Editing |
High-throughput base editing of the Bacillus subtilis genome using base editing plasmids
|
|
6 |
E. coli Genome Modification |
High-throughput knockout, insertion, or replacement of theE. coli genome using CRISPR/Cas9 technology |
|
7 |
YeastPlasmid Transformation |
High-throughput transformation of plasmids intoSaccharomyces cerevisiae |
|
8 |
E. coliPlasmid Transformation |
High-throughput transformation of plasmids intoE. coli |
|
9 |
Plasmid Extraction |
High-throughput plasmid extraction |
|
10 |
Automated Colony Picking |
Automated picking of bacterial and some fungal monoclonal colonies |
|
11 |
Automated Microbial Growth Curve Measurement |
High-throughput automated measurement of growth curves for bacteria and some fungi |
|
12 |
Automated Microbial Identification |
Rapid identification of microorganisms |
|
13 |
Automated Microbial Phenotypic Analysis |
Phenotypic chip analysis includes over 2000 types such as carbon source, nitrogen source, phosphorus source, sulfur source, osmotic pressure, pH, and antibiotics. |
|
14 |
ARTP Plasma Mutagenesis |
Conventional mutagenesis of microbial strains using atmospheric and room temperature plasma |
|
15 |
Nano-Scale Liquid Handling |
Precise ultra-micro liquid handling, range: 2.5 nL-5 µL |
|
16 |
Gene Gun |
Nucleic acid transformation of non-model microorganisms and algae |
|
17 |
Flow Analysis and Sorting |
Analysis and sorting of target cells labeled with fluorescence |
|
18 |
Droplet Microfluidics Screening System |
Selection of extracellular secretions based on fluorescence signals |
Copyright ©, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin 300308, China
Tel: 022-84861997/84861977 Fax: 022-84861926 E-mail: tib_zh(AT)tib.cas.cn
